Identification of free hydroxyl radicals in respiring rat heart mitochondria by spin trapping with the nitrone DMPO
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چکیده
منابع مشابه
EPR Detection of the Superoxide Free Radical with the Nitrone Spin Traps DMPO and BMPO
acterizing free radicals in chemistry, biology and medicine, is detection by EPR spectroscopy. However, due to their high reactivity and short half-lives, direct EPR detection of many free radicals (e.g., superoxide, hydroxyl radical, alkyl radicals, etc.) is virtually impossible in solution at room temperature. Spin trapping is a technique developed in the late 1960s in which a nitrone or nitr...
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In an ESR spin-trapping measurement of hydroxyl radicals formed in a Fenton reaction, the trapping efficiency with DMPO increased by about 300 times in a sodium trifluoroacetate solution, whereas it was little changed in a phosphate buffer.
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The aim of this study was to quantify the hydroxyl radicals (*OH) produced when aqueous solutions are decomposed by high-linear energy transfer (LET) 290 MeV/nucleon carbon-ion beams using an electron spin resonance (ESR) spectrometer. Aerated cell culture medium containing 200 mM 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was irradiated with doses of 0 to 20 Gy with an LET of 20 to 90 keV/ micro ...
متن کاملImaging free radicals in organelles, cells, tissue, and in vivo with immuno-spin trapping
The accurate and sensitive detection of biological free radicals in a reliable manner is required to define the mechanistic roles of such species in biochemistry, medicine and toxicology. Most of the techniques currently available are either not appropriate to detect free radicals in cells and tissues due to sensitivity limitations (electron spin resonance, ESR) or subject to artifacts that mak...
متن کاملFree radicals and reperfusion-induced arrhythmias: protection by spin trap agent PBN in the rat heart.
Using the isolated perfused rat heart with transient (10-minute) regional ischemia induced by coronary artery ligation, we have shown that PBN (N-tert-butyl-alpha-phenylnitrone), an organic spin trap agent designed specifically to form "stable" adducts with free radicals in electron spin resonance studies, can dramatically reduce the vulnerability of the myocardium to reperfusion-induced ventri...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)80297-9